This course will enhance your standing and expertise to prepare and successfully run samples in a metabolomic laboratory. It is designed to provide hands-on practical exercises using gas and liquid chromatography coupled to mass spectrometry (GC-MS & LC-MS). The course will focus on untargeted data acquisition in metabolomics with both nominal mass and accurate mass instruments. You will learn how to operate the instruments, how to collect metabolite profiles on complex samples and how to prepare and derivatize samples, troubleshoot instruments, acquire data and perform data quality control routines (QA/QC). Overall, you will receive comprehensive understanding of the current best practices in the metabolomics laboratory.
The course will include:
- Fundamentals of mass spectrometry, chromatography, and metabolomics.
- Hands-on exercises on sample preparation, including extraction and derivatization of complex samples for untargeted metabolomics profiling
- Hands-on exercises to learn how to operate both gas and liquid chromatography coupled to mass spectrometry (GC-MS & LC-MS).
- quality control routines (QA/QC) in order to ensure comparable data, reproducibility, and instrument performance.