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Cell lines for generation of exposome metabolites

The NIEHS-funded Compound Identification Core (CIDC) at Emory University has pioneered the use of laboratory-generated xenobiotic metabolites for use in annotation of the human exposome.  We are now making available a panel of cytochrome P450-expressing cell lines to academic researchers in metabolomics and xenobiotic metabolism.

These cell lines are prepared by lentiviral transduction of human hepatoma Huh7 cells and maintained by selection with blasticidin.  Cytochrome P450 enzymes are C-terminally tagged with the V5 peptide and can be detected or precipitated with commercially available antibodies to V5.  All listed enzymes have been verified to be catalytically active in the cells.  The lentiviral plasmids with the cloned enzymes are also available.

Recipients will be expected to provide a FedEx account number to pay for shipping.  If a large number of lines are requested, if demand is unexpectedly high, or if international shipping is required, recipients will be informed prior to shipment of any additional charges needed to recover costs. 

CYP1A2

CYP2A6

CYP2B6

CYP2C8

CYP2C9

CYP2C19

CYP2D6

CYP2E1

CYP2J2

CYP3A4

CYP3A5

CYP3A7

Send requests or enquiries to Eddie Morgan etmorga@emory.edu.  Additional cell lines are under construction or testing.

Supported by grant U2CES030163 from the National Institute for Environmental Health Sciences